Lgr5 is expressed in the cerebellum during postnatal development.

<p>Sagittal brain immunofluorescence sections from <i>Lgr5^EGFP-CreERT2</i> mice stained for EGFP to mark Lgr5+ cells at multiple time points. <b>a</b>) Tiled 10x magnified images of a midline sagittal section were stitched together to reveal Lgr5-EGFP expression at the P14 time point. <b>b</b>) Lgr5-EGFP expression at P4, P7, P10, P14, P21, P28 and P56 in the cerebellum demonstrating that Lgr5 expression turns on at P4, ramps up expression until its peak from P10-P14 and then expression is lost permanently over the next 7–14 days. <b>c</b>) Microarray expression data of Lgr5 generated from the Cerebellar Development Transcriptome Database <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114433#pone.0114433-Sato1" target="_blank">[20]</a> taken from total cerebellum RNA at indicated time points. Data acquired from <a href="http://www.cdtdb.neuroinf.jp/CDT/ReferTemporal.do?cdIdCh=CD12762.1" target="_blank">http://www.cdtdb.neuroinf.jp/CDT/ReferTemporal.do?cdIdCh=CD12762.1</a>. <b>d</b>) Olfactory Bulb (OB) at P7 and P56 reveal Lgr5-EGFP expression throughout development and adulthood. Scale bars, 400 microns.