MTs dynamics in spermatocytes.

<p><b>A</b>, Pachytene spermatocytes stained for SCP3 (red), α-Tubulin (green) and DAPI (blue). Cells were prepared without hypotonic treatment and then fixed without Triton-X100 (-). Or, cells were prepared with mild (mild extraction) or severe (severe extraction) hypotonic treatment and then fixed in Triton-X100. <b>B</b>, Single sections of spermatocytes near the nuclear periphery stained for TRF1 (red), αTubulin (green) and SCP3 (blue). Arrows indicate TRF1 signals on the MT cables. The graph indicates the average ratio of TRF1 foci on the nuclear periphery, which localize on the MT cables (379 telomeres from n = 15 zygotene cells for WT and 536 telomeres from n = 21 zygotene-like cells for <i>Sun1</i>^−/−). Error bars represent the S.D. Statistical significance (TTEST, two-tailed) was assessed (*<i>P</i><0.0005). <b>C</b>, Schematic images of meiotic chromosome movement in mammals. Bars, 5 µm.