Recombination within the <i>MAT</i> locus during α-α unisexual reproduction.

<p>A) Of the 42 genetic markers (only No. 21 to No. 42 are shown here for simplicity) that were analyzed for progeny from α-α unisexual reproduction, progeny SSB369 inherited “a” alleles from parental strain 431α for all but 6 markers (No. 31–No. 36; see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004849#pgen-1004849-t002" target="_blank">Table 2</a> for detailed marker information), which were continuous and located either within or at the edge of the <i>MAT</i> locus. The run of allele “b” stopped between markers No. 36 and No. 37 within the <i>MAT</i> locus at the 5′ end, and between markers No. 30 and No. 31 that flank the <i>MAT</i> locus at the 3′ end. The red dashed-lined rectangle indicates the <i>MAT</i> locus. B) Fine mapping conducted by sequencing the region between markers No. 36 and No. 37 showed that in progeny SSB369 the breakpoint within the <i>MAT</i> locus for the run of “b” alleles is located inside the <i>GEF1</i> gene, within a region of 150 bp in size.