Schematic of our comparative interactome-scanning pipeline.
- Xiaomu Wei (143954)
- Jishnu Das (673243)
- Robert Fragoza (673244)
- Jin Liang (1638094)
- Francisco M. Bastos de Oliveira (481529)
- Hao Ran Lee (673245)
- Xiujuan Wang (50975)
- Matthew Mort (673246)
- Peter D. Stenson (626034)
- David N. Cooper (17051)
- Steven M. Lipkin (49195)
- Marcus B. Smolka (374811)
- Haiyuan Yu (23735)
Publication date
December 2014
DOIAbstract
<p>Our pipeline begins with Clone-seq (a), a massively-parallel low-cost site-directed mutagenesis pipeline leveraging next-generation sequencing. This is followed by a high-throughput GFP assay (b) to determine protein stability, and a high-throughput Y2H assay (c), along with SILAC-based mass spectrometry (d) to determine the impact of DNA coding variants on protein interactions.</p