Cytokine response of mice immunized with peroxidoxin-1 antigen in DNA/protein prime-boost immunization regimen eight weeks post-infection.

<p>IFN-γ (A) and IL-10 (B) production in stimulated spleen cells was measured using cytokine ELISA. Mice immunized twice with pcDNA-LdPxn1 or pcDNA-mGMCSF-LdPxn1 were boosted with rLdPxn1 in the presence of CpG ODN. The control mice received three doses of pcDNA, pcDNA-mGMCSF, or CpG ODN alone. Mice were infected with stationary phase <i>L major</i> three weeks after the last immunization. Cytokine ELISA was done on antigen-stimulated and control spleen cells isolated from mice at eight weeks after infection. The data represent the mean cytokine concentration of five mice per group. Statistical comparison between groups was performed using Mann-Whitney U test. Asterisks indicate statistically significant difference in the cytokine production between mice immunized with antigen and the respective control (p<0.05).