Tests of demographic changes based on ITS2 sequences.

<p>Departure from neutrality tested using Fu’s Fs <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114039#pone.0114039-Fu1" target="_blank">[42]</a> and Ramos-Onsins and Rozas’ R₂ statistic <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114039#pone.0114039-RamosOnsins1" target="_blank">[43]</a>. Significance determined using 10,000 simulated data sets implemented by DnaSP version 5.10.01 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114039#pone.0114039-Rozas1" target="_blank">[44]</a>. τ-values estimated from demographic expansion models using ARLEQUIN v 3.11 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114039#pone.0114039-Excoffier1" target="_blank">[33]</a>. Goodness of fit tests for a model of population expansion calculated from the sum of squared deviation (SSD) and the Harpending’s raggedness index (Rag). Significance assessed by bootstrapping (10,000 replicates; ARLEQUIN v 3.11) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114039#pone.0114039-Excoffier1" target="_blank">[33]</a>.</p>$<p>All East New Zealand populations excluding Chatham;</p>‡<p>P values of (Expected SSD &gt; Observed SSD) or P (Expected Rag &gt; Observed Rag) superior to 0.05 means the null hypothesis of spatial expansion can’t be rejected;</p>#<p>nc: τ-value not calculated when spatial expansion is rejected.</p><p>Tests of demographic changes based on ITS2 sequences.