Distribution of EhCaBP5, myosin 1B and actin in <i>E. histolytica</i> during erythrophagocytosis.

<p>(A) Co- localization of EhCaBP5 and myosin 1B in <i>E. histolytica</i> cells during erythrophagocytosis. Cells were grown for 48 h and incubated with RBCs for 10 min at 37°C. The cells were then fixed and immunostained with anti- EhCaBP5 and anti-myosin 1B antibodies followed by Alexa-488 (green) and Alexa-555 (red) secondary antibodies. Arrow head depicts the co- localization of CaBP5 and myosin 1B in the phagocytic cup (upper panel) and an asterisk mark shows the absence of EhCaBP5 in the phagosome. (B) Co-localization of EhCaBP5 with F-actin. Trophozoites were stained with anti-CaBP5 antibody and TRITC-phalloidin (red) was used to stain the F-actin. The secondary antibody used for EhCaBP5 was Alexa-488 (green). Bar represents 10 µm. (DIC, differential interference contrast).