Autophagic Marker <i>MAP1LC3B</i> Expression Levels Are Associated with Carotid Atherosclerosis Symptomatology

<div><p>Objectives</p><p>The mechanism by which atheroma plaque becomes unstable is not completely understood to date but analysis of differentially expressed genes in stable versus unstable plaques may provide clues. This will be crucial toward disclosing the mechanistic basis of plaque instability, and may help to identify prognostic biomarkers for ischaemic events. The objective of our study was to identify differences in expression levels of 59 selected genes between symptomatic patients (unstable plaques) and asymptomatic patients (stable plaques).</p><p>Methods</p><p>80 carotid plaques obtained by carotid endarterectomy and classified as symptomatic (>70% stenosis) or asymptomatic (>80% stenosis) were used in this study. The expression levels of 59 genes were quantified by qPCR on RNA extracted from the carotid plaques obtained by endarterectomy and analyzed by means of various bioinformatic tools.</p><p>Results</p><p>Several genes associated with autophagy pathways displayed differential expression levels between asymptomatic and symptomatic (i.e. <i>MAP1LC3B</i>, <i>RAB24</i>, <i>EVA1A</i>). In particular, mRNA levels of <i>MAP1LC3B</i>, an autophagic marker, showed a 5−fold decrease in symptomatic samples, which was confirmed in protein blots. Immune system−related factors and endoplasmic reticulum-associated markers (i.e. <i>ERP27</i>, <i>ITPR1</i>, <i>ERO1LB, TIMP1, IL12B</i>) emerged as differently expressed genes between asymptomatic and symptomatic patients.</p><p>Conclusions</p><p>Carotid atherosclerotic plaques in which <i>MAP1LC3B</i> is underexpressed would not be able to benefit from <i>MAP1LC3B</i>−associated autophagy. This may lead to accumulation of dead cells at lesion site with subsequent plaque destabilization leading to cerebrovascular events. Identified biomarkers and network interactions may represent novel targets for development of treatments against plaque destabilization and thus for the prevention of cerebrovascular events.</p>