Enhanced MC adhesion to OP9-Dll1, -Dll4, -Jag1, or -Jag2 depended on Notch receptor-ligand interactions.

<p>An adhesion assay (60 min) for MCs on each OP9 cell (A) with EGTA (3.0 mM) or the same volume of distilled water (DW, control, 0.6% vol/vol) in a 48-well plate, (B) with 25 µg/ml of soluble recombinant DLL4-Fc, human IgG1 (control), or the same volume of PBS (19.2% vol/vol) in a 96-well plate, (C) with 50 µg/ml of anti-Dll1 mAb, anti-CTLA4 mAb (control), or the same volume of PBS (5.0% vol/vol) in a 96-well plate, (D) with 200 µg/ml of anti-Dll4 mAb, anti-CTLA4 mAb (control), or the same volume of PBS (20.0% vol/vol) in a 96-well plate. Data represent the percentages of non-adherent MCs (mean ± SEM of triplicate cultures) (*p<0.05 significantly different from each control treatment on the same OP9 cells, the Student’s <i>t-</i>test).