TLC analysis of FOS from transglycosylation reactions.

<p>The transglycosylation reactions were catalyzed by native and recombinant BfrAs incubated with 33% sucrose at optimal reaction condition for 5 h. The FOS were separated by Bio-Gel P2 column. SD, FOS standards; Glc, glucose; Fru, fructose; Suc, sucrose; NK, neokestose; NA, transglycosylation reaction catalyzed by N.BfrA; P, transglycosylation reaction catalyzed by P.BfrA; E, transglycosylation reaction catalyzed by E.BfrA. The neokestose (NK), 6-kestose (6-K), and 6-nystose (6-N) were indicated by blue, red, and dark arrows, respectively.