The kinetic parameters for the synthetic reactions catalyzed by Teth514_1788 and Teth514_1789.

a<p>The kinetic parameters were calculated by fitting the initial velocities to various concentrations of acceptor substrates in the presence of 10 mm α-Man1<i>P</i> using the Michaelis-Menten equation.</p>b,c<p>The kinetic parameters were calculated by fitting the initial velocities toward various concentrations of donor substrates in the presence of 10 mm β-1,2-Man₂ (<i>b</i>) or 10 mmd-mannose (<i>c</i>) using the Michaelis-Menten equation.</p>d<p>Not detectable. To investigate the acceptor specificities, the synthetic reactions were examined using the following putative carbohydrate acceptors: d-allose, d-altrose, d-arabinose, d-fructose, d-galactosamine, d-galactose, d-galacturonic acid, d-glucosamine, d-glucose, d-glucuronic acid, d-lyxose, d-talose, d-ribose, d-xylose, l-arabinose, l-rhamnose, 1,5-anhydro-d-glucitol, 2-deoxy-d-glucose, methyl-α-d-glucoside, methyl-β-d-glucoside, 3-<i>O</i>-methyl-d-glucose, <i>N</i>-acetyl-d-galactosamine, <i>N</i>-acetyl-d-glucosamine, <i>N</i>-acetyl-d-mannosamine, cellobiose, gentiobiose, isomaltose, kojibiose, lactose, lactulose, laminaribiose, maltose, melibiose, nigerose, <i>N</i>,<i>N</i>′-diacetylchitobiose, sophorose, sucrose, trehalose, and xylobiose.</p><p>The kinetic parameters for the synthetic reactions catalyzed by Teth514_1788 and Teth514_1789.