Triple-transgenic thymocytes exhibit efficient allelic exclusion of TCR Vα chains.

<p>(A) Double-negative (DN) and CD4 and CD8 single positive (SP) thymocytes from B6 (top), Vα2 Vα3.2 (middle) and Vα2 Vα3.2 Vβ5 (bottom) mice were analyzed for surface expression of Vα2, Vα3.2 and Vβ5 in TCR^int and TCR^hi gate. (B) DN and CD4 and CD8 SP thymocytes from B6 (top), Vα2 Vα3.2 (middle) and Vα2 Vα3.2 Vβ5 (bottom) mice were analyzed for intracellular expression of Vα2, Vα3.2 and Vβ5 in TCR^int and TCR^hi gate. FACS plots representative of >10 mice per genotype and are derived from the same dataset as <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0114320#pone-0114320-g004" target="_blank">Fig. 4</a>.