<i>P. gingivalis</i> LPS-induced TSP-1 mRNA expression was inhibited by TLR2-neutralizing antibody in THP-1 cells.

<p>THP-1 cells were pretreated with 10 ng/ml of IgG2a isotype control, TLR2-neutralizing antibody, and TLR4-neutralizing antibody for 30 min, followed by the addition of 1.0 µg/ml of <i>P. gingivalis</i> LPS, Pam2CSK4, and <i>E. coli</i> LPS for 4 h. (A) TLR2-neutralizing antibody and TLR4-neutralizing antibody neutralized the activity of Pam2CSK4 and <i>E. coli</i> LPS, respectively. (B) <i>P. gingivalis</i> LPS with TLR2-neutralizing antibody significantly reduced TSP-1 mRNA expression compared with <i>P. gingivalis</i> LPS alone in THP-1 cells (<i>P</i><0.001).