The <i>in vitro</i> inhibition of IgG-antibodies binding to H1N1 antigen of Pandemrix is evaluated using H1N1 antigen suspension of Pandemrix, Arepanrix or detergents present in Pandemrix as liquid-phase inhibitors pre-incubated with plasma samples from study subjects. A.

<p>The inhibition of IgG-antibodies binding to solid-phase bound H1N1 antigen of Pandemrix (% of inhibition in y-axis) is lower when Arepanrix antigen (1/10) is used as inhibitor compared to Pandemrix (1/10) as an inhibitor both in children with narcolepsy (N; n = 47) and in healthy vaccinated children (C; n = 57). <b>B.</b> The inhibition of IgG-antibodies binding to solid-phase bound H1N1 antigen of Pandemrix (% of inhibition in y-axis) with Arepanrix antigen(1/10) is weaker in the children with narcolepsy who are homozygotic for HLA DQB1*06∶02 risk allele than in the children with narcolepsy or healthy children who are heterozygotic for HLA DQB1*06∶02 risk allele of narcolepsy. <b>C.</b> Dose-dependent inhibition of IgG-antibodies binding to solid-phase bound H1N1 antigen of Pandemrix (% of inhibition in y-axis) using as a liquid phase inhibitor H1N1 antigen suspension of Arepanrix or Pandemrix at the final dilutions of 1/250, 1/50 and 1/10 (0.4, 2, 10 on x-axis) or the detergents present in the H1N1 antigen suspension of Pandemrix, Triton X and polysorbate 80, at the final concentrations 0.8, 4, and 20 µg/mL (0.4, 2, 10 on axis). The inhibition curves represent mean value of the % of inhibition in plasma samples from 3 children with narcolepsy.