Antibodies to untreated and detergent treated recombinant viral proteins studied by EIA.

<p>IgG-antibodies against untreated recombinant viral proteins, rHA (A) and rNP (B), and against rHA and rNP exposed to non-ionic detergents, Triton X and polysorbate 80 (PS80), used in the manufacturing process of Pandemrix H1N1 antigen, but not Arepanrix, in six plasma samples from vaccinated children with narcolepsy C. Children with narcolepsy (N; n = 47) had higher levels of IgG-antibodies to polysorbate 80 treated recombinant NP (PS80) but not to untreated NP (PBS) than healthy vaccinated children (C; n = 57). Children with narcolepsy who all carry HLA DQB1*06∶02 allele (N; n = 47) and healthy vaccinated children with HLA DQB1*06∶02 allele (C/DQ6+; n = 20)) had higher levels of antibodies to untreated NP in comparison to the children without HLA DQB1*06∶02 allele (C/DQ6−; n = 37). D. Children with narcolepsy (N; n = 47) showed higher levels of IgG-antibodies to untreated recombinant HA (PBS) and polysorbate 80 treated HA (PS80) than healthy vaccinated children (C; n = 57). No difference was found in the antibody levels to treated or untreated HA between healthy children with or without HLA DQB1*06∶02 allele (C/DQ6+; n = 20 and C/DQ6−; n = 37).