Energy metabolism and oxidative stress upon incubation with etomoxir, 2-DG and ATO in HL60 cells.

<p>(A, B) Relative fraction of ATP in relation to total nucleotide (ATP+ADP+AMP) content (A), and AMP/ATP ratio (B), in untreated cells (Cont) and cells incubated for 6 h with the indicated concentrations of 2-DG and etomoxir, alone and in combination, and in the presence or absence of ATO. For more complete information see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115250#pone.0115250.s005" target="_blank">S1 Table</a>. (C, D) Changes in intracellular ROS accumulation (C) and GSH content (D), in cells incubated for 4 h with etomoxir and 2-DG, alone or in combination, and in the presence or absence of ATO. 3-BrP (60 µM) was used as a positive control in D (see Ref. 23). The values represent the mean ± S.D. of at least five determinations. Symbols in A–C indicate significant differences in relation to the control or between the indicated pairs of treatments. Symbols in D indicate significant differences in relation to the control (n.s., non-significant). ATO was always used at 2 µM. For other conditions, see legends of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115250#pone-0115250-g001" target="_blank">Figs. 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115250#pone-0115250-g004" target="_blank">4</a>.