The effects of ASIC channel blockers on ASIC currents in freshly isolated anterior pituitary cells.

<p><b>A</b>. Representative ASIC currents elicited by pH 5.5 with various concentrations of amiloride. Holding potential was −70 mV. The cells were held at pH 7.4 before and in between applications of pH 5.5. Amiloride was applied during acid application. <b>B</b>. Summary data showing the dose-dependent block of ASIC currents by amiloride in anterior pituitary cells (n = 7). Currents were normalized to the control condition (without amiloride). The IC₅₀ of amiloride block was 6.3±1.0 µM (n = 7). C. Representative ASIC currents in WT and ASIC2^-/- cells elicited by pH 5.5 with 100 nM PcTx1. Cells were pre-treated with PcTx1 in pH 7.4 for 1 min and then were perfused with pH 5.5 containing 100 nM PcTx1. Holding potential was −70 mV. D. Summary data showing the block of ASIC currents by 100 nM PcTx1 in anterior pituitary cells from WT (n = 7) and ASIC2^-/- mice (n = 6). * <i>P</i><0.05 compared to control, Student's <i>t</i>-test.