Effect of suppressed HIF-1A expression on arsenite mediated transformation.

<p>A) Immunoblot analysis of HIF-1A knockdown in BEAS-2B, short immunoblot exposure shown for MG132-treated samples; long immunoblot exposure shown for MG132-untreated samples. B) QPCR for HIF-1A mRNA. Bars represent mean, +1 standard deviation, from 5 experimental replicates. C) Lactate levels (percent control) in arsenite-exposed (denoted “As”, exposed for 8 weeks) and unexposed control (denoted “Ct”) BEAS-2B stably transfected with scrambled control shRNA (denoted “Vector”) or with shRNA targeting HIF1A (denoted “shHIF1A”) expression. Absolute lactate production in vector control: 0.696±0.04 µmol/10⁶cells/hr). Bars represent mean, +1 standard deviation, from 3 experimental replicates. D) Colony count of soft agar assay from BEAS-2B cells treated as described above in panel C. Bars represent mean, +1 standard deviation, from 3 experimental replicates. *p<0.05.