Glycolysis induction by HIF-1A overexpression in BEAS-2B.

<p>A) Immunoblot analysis of HIF-1A in BEAS-2B, vector control and transiently transfected with degradation-resistant HIF-1A mutant. B) Lactate levels (percent vector control) in cells described in 2A (Absolute lactate production in vector control: 0.729±0.054 µmol/10⁶cells/hr). Bars represent mean, 1 standard deviation, from 3 independent replicates. *p<0.05. C) Intracellular metabolite concentration (percent control BEAS-2B) of 1 µM arsenite-exposed (2 weeks) BEAS-2B cells. Bars represent mean, 1 standard deviation, from 4 experimental replicates. For each metabolite, levels in arsenite-exposed BEAS-2B are significantly different compared to control (p<0.05).