Expression of LOX-1, VEGF VEGFR2, HIF-1α, Nox2 and generation of ROS.

<p><b>A. Effect of ischemia on expression of LOX-1.</b> a: The expression of LOX-1 was analyzed in the ischemic/nonischemic hindlimb of WT mice by Western blotting. Upregulation of LOX-1 expression in the ischemic hindlimb tissue on postoperative day 7 after ligation compared with in the nonischemic hindlimb of WT mice. Actin was used as loading control. IH: ischemia. b: Densitometric measurements (mean±SD) were performed to evaluate the fold increase in LOX-1 protein. The measured value is expressed as a value relative to the protein level of WT mice. IH: ischemia, ^★, P<0.05 vs. WT, n = 4. <b>B. Effect of deletion of LOX-1 on expression of VEGF, VEGFR2, HIF-1α, Nox2 and generation of ROS.</b> a: The expression of VEGF, VEGFR2, HIF-1α and Nox2 in the ischemic hindlimb was analyzed by Western blotting. Suppression of VEGF, HIF-1α and Nox2 expression in the ischemic hindlimb tissue of LOX-1 KO mice on postoperative day 7 after ligation compared with that in WT mice. Actin was used as loading control. b: Densitometric measurements (mean±SD) were performed to evaluate the fold increase in VEGF, VEGFR2, HIF-1α and Nox2 protein. The measured value is expressed as a value relative to the protein level of WT mice. ^★, P<0.01 vs. WT, n = 4. c: ROS generation was measured in the ischemic hindlimb of WT mice and LOX-1 KO mice. ^★, P<0.01 vs. WT, n = 4.