Central role of the vacuole in cysteine catabolism to H₂S.

<p><b>A</b> Extracellular release of cysteine-generated H₂S (upper panel) and biomass formation (lower panel) profiles of <i>wt</i> and mutant in vacuole biogenesis (<i>Δvam1</i>). Experiments were carried out in quadruplicates. <b>B</b> Extracellular release of cysteine-generated H₂S for mutants deleted in each of the sub-units comprising V₁ of the V-ATPase complex. Experiments were carried out in quadruplicates. <b>C</b><i>in-vivo</i> downregulation of the yeast V-ATPase complex through nutrient limitation. H₂S generation profile measured with H₂S detection tubes (left panel) and biomass formation profile (right panel) for BY4742 cells grown with glucose or galactose as carbon source. An addition of glucose to a final concentration of 0.112 M was made to the galactose medium at the time indicated by arrows.