Generation of ES cell reporter cell line with multiple fluorescence proteins co-expression.

<p>(A) Schematic representation of inducible fluorescence protein expressing cassettes in the context of <i>PiggyBac</i> backbone. (B) Imaging of generated individual fluorescence reporter under different excitation light. Note that mAmetrine fluorescence was photographed under blue light thus has weak signal. The scale bar is 200 µm. (C) Genotyping analysis of generated ES cell reporter with five fluorescence proteins coexisted in single ES cell. (D) Flow analysis of generated No.12 multiple fluorescence protein stable ES cells. The thin red line on the left is the histogram without doxycline treatment as the negative control.