Efficient neural differentiation of TW1 hESCs.

<p>(A) The Oct4 transcription factor, a well-characterized pluripotent marker, was detected in undifferentiated TW1 hESCs. (B) Dissociated hESCs aggregated in suspension and formed numerous embryonic bodies. (C, D) Differentiating hESCs exhibited reduced expression of Oct4 on D5 (C) and displayed a classical neural rosette conformation after plating on culture plates on D10 (D). (E–G) NPCs express N-cadherin (E), nestin (F), Pax6 and Sox1 transcription factors (G). (H) Extensive TuJ1⁺ neurites were detected in NPC-derived mature neurons on D25. Scale bars, 100 µm.