Changes in cell viability and necrosis of shrimp hemocytes incubated in EM-C, EM-L, EM-β, and EM-P, and percentage of necrotic cell of different EMs.

<p>(A) Viable and nonviable cells are designated in marker 3 (M3) and marker 4 (M4), respectively. Changes in viability over time of shrimp hemocytes showing the kinetics and proportion of viable hemocyte cells incubated in EM-C, EM-L, EM-β, and EM-P. Cell viability of shrimp hemocytes receiving shrimp salt solution (SSS) served as the control group. (B–E) Fluorescence micrographs and light micrographs showing necrosis of shrimp hemocytes incubated in EM-C (B), EM-L (C), EM-β (D), and EM-P (E) for 30 min. (F) Percentage of necrotic cells of shrimp hemocytes incubated in EM-C, EM-L, EM-β, and EM-P for 30 min. Each bar represents the mean value from six shrimp with standard error. Data with different letters significantly differ (<i>p</i> <0.05) among treatments. The percentages of necrotic cells of shrimp hemocytes incubated in EM-L, EM-β, and EM-L were significantly higher than in EM-C. See Fig. 2 for the abbreviation of EM-C, EM-L, EM-β, and EM-P. N, Nucleus; NL, Nuclei lysed (dead cells); Scale = 20 µm.