EBV viral loads and impact of EBV co-infection on survival in HIV-infected infants.

<p>A: Infants with congenital EBV had viral loads measured at birth (within 96 hours of delivery) and at 6 weeks of age. Viral loads were measured following viral nucleic acid extraction from 100 uL plasma by real-time PCR, using the EBV Artus LC kit (Qiagen) on a LightCycler 2.0 (Roche) machine, with limit of detection of 5.78 copies/uL (equivalent to 2890 copies/mL; shown as dotted line labeled LOD) and limit of quantification of 10 copies/uL (equivalent to 5000 copies/mL; shown as dotted line labeled LOQ). Infants with low positive results (above LOD but below LOQ) are shown arbitrarily as having a result halfway between the LOD and LOQ. B: Survival among infants with postnatal EBV infection (negative EBV PCR at birth, positive EBV PCR at 6 weeks of age) or without EBV co-infection (negative EBV PCR at 6 weeks of age), from time of viral testing (6 weeks of age) through 6 months of age. Adjusted hazard ratio from Cox proportional hazards model is shown, adjusting for maternal education, CD4 count and death, and infant gender, low birth weight and 6-week HIV viral load.