Autophagy is activated by 5-FU treatment and starvation in HT29 cells.

<p>HT29 cells were treated with 5 µM of 5-FU or not. Activation of autophagy was observed by LC3 immunofluorescence (A). HT29 cells were starved in Krebs-Ringer buffer or not. Activation of autophagy was observed by LC3 immunofluorescence (B). DAPI staining was performed for identifying nucleus. LC3, p62 and mTOR immunoblotting was performed using the lysates of HT29 cells treated by 5 µM of 5-FU for 24 h or not, and starved for 7 h or not (C). Data are the representative of three independent experiments. Bar, 20 µm.