Validation of changes in AC components observed by MS.

<p>A) Isolated ACs were probed by Western blotting for α_v-integrin, tensin-1, filamin A, ELKS and PDLIM5. B) To investigate the pattern of changes to integrins upon loss of microtubules further, HFFs treated with DMSO or nocodazole (Noc) were fixed and immunostained for α₅-integrin, α_vβ₃-integrin and vinculin and quantified as a percentage of the total cell area (line, median; box, interquartile range; whiskers, maximum and minimum; ****<i>p</i><0.0001, Student's <i>t</i>-test; <i>n</i> = 54). C) Immunostained cells showing the cellular distribution of α₅-integrin (red), α_vβ₃-integrin (green) and vinculin (blue). Blue and yellow insets are 4× enlargements of corresponding boxes.