Changes to actin stress fibres and vinculin-containing ACs upon microtubule disruption.

<p>Serum starved HFFs were treated with DMSO and nocodazole (Noc) for 4 hours and fixed either with A) paraformaldehyde supplemented with glutaraldehyde to preserve microtubules, and immunostained for actin (green), vinculin (red) and tubulin (blue) or with B) paraformaldehyde and immunostained for vinculin. Vinculin staining was quantified as a percentage of the total cell area and displayed as a Beeswarm-Boxplot (line, median; box, interquartile range; whiskers, maximum and minimum; <i>n</i> = 51 to 55 cells).