Result of mutagenesis on the molar conversion efficiency and time profile for product formation.

Fig 5 shows specific activity (both forward and reverse reactions) of the wild type PfMDH, E18Q, V190W, E18W mutants, and co-purified WT-mutant chimeric complex.

Sergey Lunev (5142905)
Sabine Butzloff (5142896)
Atilio R. Romero (5142899)
Marleen Linzke (5142902)
Fernando A. Batista (5142893)
Kamila A. Meissner (5142908)
Ingrid B. Müller (43367)
Alaa Adawy (1609960)
Carsten Wrenger (37474)
Matthew R. Groves (43369)

April 2018

Specific activity is shown in Units per mg of enzyme (U mg-1). U = μmol of NADH oxidiz...

Enhancement of the NAD(P)(H) pool in Escherichia coli for biotransformation

Heuser, F.
Schroer, K.
Lütz, S.
Bringer-Meyer, S.
Sahm, H.

January 2007

In pyridine nucleotide-dependent, reductive whole cell biotransformation with resting cells of Esche...

Protein Engineering by In Vivo Incorporation of Non-Natural Amino Acids: Control of Incorporation of Methionine Analogues by Methionyl-tRNA Synthetase

Kiick, Kristi L.
Tirrell, David A.

November 2000

The incorporation of non-natural amino acids is an important strategy for engineering novel chemical...

Apparent kinetic parameters of KMTB production using wild type and mutant l-AAD containing whole-cell biocatalysts.

Gazi Sakir Hossain (678022)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Guocheng Du (390526)
Miao Wang (248377)
Long Liu (390523)
Jian Chen (15340)

December 2014

aThe volume of each reaction mixture was 20 ml, and amount of biocatalyst in the reaction solutio...

One-step biosynthesis of α-keto-γ-methylthiobutyric acid from L-methionine by an Escherichia coli whole-cell biocatalyst expressing an engineered L-amino acid deaminase from Proteus vulgaris.

Gazi Sakir Hossain
Jianghua Li
Hyun-dong Shin
Guocheng Du
Miao Wang
Long Liu
Jian Chen

α-Keto-γ-methylthiobutyric acid (KMTB), a keto derivative of l-methionine, has great potential for u...

One-Step Biosynthesis of α-Keto-γ-Methylthiobutyric Acid from L-Methionine by an Escherichia coli Whole-Cell Biocatalyst Expressing an Engineered L-Amino Acid Deaminase from Proteus vulgaris

Gazi Sakir Hossain (678022)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Guocheng Du (390526)
Miao Wang (248377)
Long Liu (390523)
Jian Chen (15340)

December 2014

<div>α-Keto-γ-methylthiobutyric acid (KMTB), a keto derivative of l-methionine, has great potenti...

The effect of mutants in 5’-gene sequences on L-AAD catalyst activity, cell growth and KIC production.

Yang Song (1235364)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Long Liu (390523)
Guocheng Du (390526)
Jian Chen (15340)

June 2017

a and b: The effect of mutant at 5’-gene sequences on biocatalyst activity and cell...

Efficient cell factories for the production of N-methylated amino acids and for methanol-based amino acid production

Irla, Marta
Wendisch, Volker F.

January 2022

Irla M, Wendisch VF. Efficient cell factories for the production of N-methylated amino acids and for...

Designing an optmized met X/Y enzymes for an efficient methionine production in E.coli

Rodrigues, Luísa

January 2022

Methionine is a proteinogenic amino acid critical amino for the pharmaceutical, biotechnological and...

The effect of ribosome binding site design on L-AAD catalyst activity, cell growth and KIC production.

Yang Song (1235364)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Long Liu (390523)
Guocheng Du (390526)
Jian Chen (15340)

June 2017

a: The comparison of biocatalyst activity with predicted translation rate. Gray bar: the b...

Development of efficient microbial cell factory for whole-cell bioconversion of L-threonine to 2-hydroxybutyric acid

Le, Thai
Park, Sunghoon

January 2022

Production of 2-hydroxybutyric acid (2-HBA) was attempted in recombinant Escherichia coli W3110 Atdh...

Characterization of a mutation demonstrating a link between the lack of S-adenosylmethionine and cell division in Escherichia coli

Budman, Lorne

January 1998

The metK gene of Escherichia coli encodes for the enzyme S-adenosylmethionine (SAM) synthetase, whic...

Catalytic performance of engineered double mutant l-AAD containing E. coli cells immobilized in various matrices.

Gazi Sakir Hossain (678022)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Guocheng Du (390526)
Miao Wang (248377)
Long Liu (390523)
Jian Chen (15340)

December 2014

Conditions: 20 mL Tris-HCl buffer (50 mM, pH 8.0), with optimum amount of free cells or immobiliz...

Colony number and mutation rate obtained by hydroxyl amine mediated mutagenesis.

Anke Bill (315423)
Elizabeth M. Rosethorne (571074)
Toby C. Kent (571075)
Lindsay Fawcett (571076)
Lynn Burchell (327295)
Michiel T. van Diepen (571077)
Anthony Marelli (571078)
Sergey Batalov (571079)
Loren Miraglia (266334)
Anthony P. Orth (105751)
Nicole A. Renaud (170992)
Steven J. Charlton (571080)
Martin Gosling (571081)
L. Alex Gaither (548263)
Paul J. Groot-Kormelink (360500)

June 2014

Colony number and mutation rate obtained by hydroxyl amine mediated mutagenesis.</p

Escherichia coli Deletion Mutants Illuminate Trade-Offs between Growth Rate and Flux through a Foreign Anabolic Pathway

Kelly C. Falls (519915)
Aimee L. Williams (519916)
Anton V. Bryksin (361159)
Ichiro Matsumura (361160)

February 2014

<div>Metabolic engineers strive to improve the production yields of microbial fermentations, some...

Fig 5 shows specific activity (both forward and reverse reactions) of the wild type PfMDH, E18Q, V190W, E18W mutants, and co-purified WT-mutant chimeric complex.

Sergey Lunev (5142905)
Sabine Butzloff (5142896)
Atilio R. Romero (5142899)
Marleen Linzke (5142902)
Fernando A. Batista (5142893)
Kamila A. Meissner (5142908)
Ingrid B. Müller (43367)
Alaa Adawy (1609960)
Carsten Wrenger (37474)
Matthew R. Groves (43369)

April 2018

Specific activity is shown in Units per mg of enzyme (U mg-1). U = μmol of NADH oxidiz...

Enhancement of the NAD(P)(H) pool in Escherichia coli for biotransformation

Heuser, F.
Schroer, K.
Lütz, S.
Bringer-Meyer, S.
Sahm, H.

January 2007

In pyridine nucleotide-dependent, reductive whole cell biotransformation with resting cells of Esche...

Protein Engineering by In Vivo Incorporation of Non-Natural Amino Acids: Control of Incorporation of Methionine Analogues by Methionyl-tRNA Synthetase

Kiick, Kristi L.
Tirrell, David A.

November 2000

The incorporation of non-natural amino acids is an important strategy for engineering novel chemical...

Apparent kinetic parameters of KMTB production using wild type and mutant l-AAD containing whole-cell biocatalysts.

Gazi Sakir Hossain (678022)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Guocheng Du (390526)
Miao Wang (248377)
Long Liu (390523)
Jian Chen (15340)

December 2014

aThe volume of each reaction mixture was 20 ml, and amount of biocatalyst in the reaction solutio...

One-step biosynthesis of α-keto-γ-methylthiobutyric acid from L-methionine by an Escherichia coli whole-cell biocatalyst expressing an engineered L-amino acid deaminase from Proteus vulgaris.

Gazi Sakir Hossain
Jianghua Li
Hyun-dong Shin
Guocheng Du
Miao Wang
Long Liu
Jian Chen

α-Keto-γ-methylthiobutyric acid (KMTB), a keto derivative of l-methionine, has great potential for u...

One-Step Biosynthesis of α-Keto-γ-Methylthiobutyric Acid from L-Methionine by an Escherichia coli Whole-Cell Biocatalyst Expressing an Engineered L-Amino Acid Deaminase from Proteus vulgaris

Gazi Sakir Hossain (678022)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Guocheng Du (390526)
Miao Wang (248377)
Long Liu (390523)
Jian Chen (15340)

December 2014

<div>α-Keto-γ-methylthiobutyric acid (KMTB), a keto derivative of l-methionine, has great potenti...

The effect of mutants in 5’-gene sequences on L-AAD catalyst activity, cell growth and KIC production.

Yang Song (1235364)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Long Liu (390523)
Guocheng Du (390526)
Jian Chen (15340)

June 2017

a and b: The effect of mutant at 5’-gene sequences on biocatalyst activity and cell...

Efficient cell factories for the production of N-methylated amino acids and for methanol-based amino acid production

Irla, Marta
Wendisch, Volker F.

January 2022

Irla M, Wendisch VF. Efficient cell factories for the production of N-methylated amino acids and for...

Designing an optmized met X/Y enzymes for an efficient methionine production in E.coli

Rodrigues, Luísa

January 2022

Methionine is a proteinogenic amino acid critical amino for the pharmaceutical, biotechnological and...

The effect of ribosome binding site design on L-AAD catalyst activity, cell growth and KIC production.

Yang Song (1235364)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Long Liu (390523)
Guocheng Du (390526)
Jian Chen (15340)

June 2017

a: The comparison of biocatalyst activity with predicted translation rate. Gray bar: the b...

Development of efficient microbial cell factory for whole-cell bioconversion of L-threonine to 2-hydroxybutyric acid

Le, Thai
Park, Sunghoon

January 2022

Production of 2-hydroxybutyric acid (2-HBA) was attempted in recombinant Escherichia coli W3110 Atdh...

Characterization of a mutation demonstrating a link between the lack of S-adenosylmethionine and cell division in Escherichia coli

Budman, Lorne

January 1998

The metK gene of Escherichia coli encodes for the enzyme S-adenosylmethionine (SAM) synthetase, whic...

Catalytic performance of engineered double mutant l-AAD containing E. coli cells immobilized in various matrices.

Gazi Sakir Hossain (678022)
Jianghua Li (390525)
Hyun-dong Shin (390524)
Guocheng Du (390526)
Miao Wang (248377)
Long Liu (390523)
Jian Chen (15340)

December 2014

Conditions: 20 mL Tris-HCl buffer (50 mM, pH 8.0), with optimum amount of free cells or immobiliz...

Colony number and mutation rate obtained by hydroxyl amine mediated mutagenesis.

Anke Bill (315423)
Elizabeth M. Rosethorne (571074)
Toby C. Kent (571075)
Lindsay Fawcett (571076)
Lynn Burchell (327295)
Michiel T. van Diepen (571077)
Anthony Marelli (571078)
Sergey Batalov (571079)
Loren Miraglia (266334)
Anthony P. Orth (105751)
Nicole A. Renaud (170992)
Steven J. Charlton (571080)
Martin Gosling (571081)
L. Alex Gaither (548263)
Paul J. Groot-Kormelink (360500)

June 2014

Colony number and mutation rate obtained by hydroxyl amine mediated mutagenesis.</p

Escherichia coli Deletion Mutants Illuminate Trade-Offs between Growth Rate and Flux through a Foreign Anabolic Pathway

Kelly C. Falls (519915)
Aimee L. Williams (519916)
Anton V. Bryksin (361159)
Ichiro Matsumura (361160)

February 2014

<div>Metabolic engineers strive to improve the production yields of microbial fermentations, some...

Fig 5 shows specific activity (both forward and reverse reactions) of the wild type PfMDH, E18Q, V190W, E18W mutants, and co-purified WT-mutant chimeric complex.

Sergey Lunev (5142905)
Sabine Butzloff (5142896)
Atilio R. Romero (5142899)
Marleen Linzke (5142902)
Fernando A. Batista (5142893)
Kamila A. Meissner (5142908)
Ingrid B. Müller (43367)
Alaa Adawy (1609960)
Carsten Wrenger (37474)
Matthew R. Groves (43369)

April 2018

Specific activity is shown in Units per mg of enzyme (U mg-1). U = μmol of NADH oxidiz...

Enhancement of the NAD(P)(H) pool in Escherichia coli for biotransformation

Heuser, F.
Schroer, K.
Lütz, S.
Bringer-Meyer, S.
Sahm, H.

January 2007

In pyridine nucleotide-dependent, reductive whole cell biotransformation with resting cells of Esche...

Protein Engineering by In Vivo Incorporation of Non-Natural Amino Acids: Control of Incorporation of Methionine Analogues by Methionyl-tRNA Synthetase

Kiick, Kristi L.
Tirrell, David A.

November 2000

The incorporation of non-natural amino acids is an important strategy for engineering novel chemical...

Result of mutagenesis on the molar conversion efficiency and time profile for product formation.

Abstract

Extracted data

Result of mutagenesis on the molar conversion efficiency and time profile for product formation.

Abstract

Extracted data

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