The decreased survival of quiescent cells deprived of Sub1 does not depend on strain genotypes or medium composition.

<p>(<b>A</b>) Two sets of the indicated strains generated in YPH500 with <i>sub1Δ::kanMX6</i> and <i>maf1Δ::ura3</i> deletion cassettes (Set 1) or with <i>sub1Δ::his3</i> and <i>maf1Δ::kanMX6</i> deletion cassettes (Set 2) were grown to stationary phase in YPD, SC or SD4x medium. Samples of cultures were spotted onto YPD plates at the indicated day (d1–d10 for SC or SD4x; d5–d25 for YPD) and the relative growth of the strains was observed after 4 days of incubation at 30°C. (<b>B</b>) Viability was determined over time by counting the number of colony forming units (Cfu) per volume of culture. Life-span curves represent means and standard deviations from at least two independent experiments. Note that life span curves obtained with the second set of strains in SC medium are similar to those in SD4x medium for the first set shown here. (<b>C</b>) Viability of the indicated strains grown in SC medium was determined over time by trypan blue exclusion assays. Viability curves represent means and standard deviations from three independent experiments.