Strategy of analysis of Flow Cytometer data from one representative experiment with PBMC samples of HIV infected children.

<p>(A) Were used high Quality Parameters: (Time x PE-to check laser status; Singlets-to exclude the doublets cells; SSC x FSC- to choose lymphocytes subset; Live/Dead-to exclude the dead cells; gate SSC x CD3- to chose T lymphocytes and gate CD4 x CD8- to separate in CD4 and CD8 T subsets, then b Naïve/Memory subsets and cellular activation were evaluated in CD4 T Cells and CD8 T Cells, (B) Naïve/Memory CD4⁺ Subsets were classified through differentiated expression of three markers (CD45RA, CD27 and CCR7): 1- Naïve (CD45RA⁺CD27⁺CCR7⁺); 2- Central Memory (CD45RA⁻CD27⁺CCR7⁺); 3- Transitory Memory (CD45RA⁻CD27⁺CCR7⁻); 4- Intermediary Memory (CD45RA⁺CD27⁺CCR7⁻); 5- Effector Memory (CD45RA⁻CD27⁻CCR7⁻); 6- Effector (CD45RA⁺CD27⁻CCR7⁻); Cellular Activation were characterized through expression of CD38, HLA-DR and CCR5.