<i>E. coli</i> growth curves.

<p>(A) The detection limit of RFD-EC1 for <i>E. coli</i> measured by dPAGE analysis of 2 pmol RFD-EC1 following 1-h incubation in 50 µL of reaction buffer (1× RB) containing 10, 100, 10³, 10⁴, 10⁵, 10⁶, 10⁷ or 10⁸<i>E. coli</i> cells. (B) <i>E. coli</i> growth curves measured by RFD-EC1 and optical density method with 100 seeding cells. NC = negative control (no <i>E. coli</i>), Marker = RFD-EC1 treated in 2 M NaOH at 90°C for 10 min. (C) Tracking <i>E. coli</i> cells growth by RFD-EC1 in microwell plate. The culture time was 0, 1, 4, 8, 12 hours, and the number of seeding cells was 10, 10², 10³, 10⁴ and 10⁵ respectively. 50 µL from each 200-µL culture was used for the cleavage reactions.