BxPC-3 cells deficient of both β-catenin and plakoglobin cannot form normal cell to cell connections.

<p>A) Quantification of plakoglobin protein levels in wild type and gene disrupted cells after transfection with control or plakoglobin siRNA. Cell lysates were harvested 72 hours after transfection and analyzed by quantitative WB. The values are normalized so that the levels of plakoglobin protein in untransfected cells are set to 100%. B) Morphology of untransfected, control and plakoglobin siRNA transfected wild type and gene disrupted clones. Phase contrast images were acquired 72 hours after siRNA transfection. C) Quantification of apoptosis levels in untransfected, control and plakoglobin siRNA transfected wild type and gene disrupted clones. Shown are the percentage AnnexinV-FITC positive cells as determined by flow cytometry. Error bars represent the SD between three parallels.