Acute ethanol treatment increased expression of BNIP3 and NIX in primary hepatocytes and mouse liver.

<p>Primary cultured mouse hepatocytes were treated with ethanol (80 mM) for 6 hours. Densitometry was performed and data are presented as a ratio vs control (n = 3). (<b>A</b>) Total cell lysates from cultured hepatocytes were subjected to western blot analysis. (<b>B</b>) Male C57BL/6 mice were treated with either water or ethanol (4.5 g/kg) by gavage for 6, 12 or 16 hours. Hepatic mRNA was isolated and real-time RT-PCR was performed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115849#s2" target="_blank">Materials and Methods</a>. Data are presented as means ± SE (n = 4–6). * p<0.05, vs 0 hour control. One way ANOVA with Scheffé's post hoc test. (<b>C</b>) Mice were treated as in (<b>B</b>) and total liver lysates from the mice (16 hours treatment) were subjected to western blot analysis. Densitometry was performed and data are presented as a ratio of control (n = 3).