Characterization of MeCP2^R168X/y mice.

<p>Analysis of survival included 71 mutant mice (A). Body weight was measured weekly and compared to wild type littermates (B). Occurrence of hind limb clasping was measured weekly (C). Nest building was analyzed after 24 and 48 hours and scored according to Deacon 2006. Data were shown as box plots with median (−), mean (+) and whiskers indicating 5–95 percentile. (D). Plethysmography (E) was performed to analyze occurrence of apnea (F) as well as breathing pattern including the frequency (G) and the irregularity score (IrrScore) (H). Rotarod was used to analyze motor coordination. Latency to fall was measured on 2 consecutive days (T1–T4) at constant speed (I) and on 2 consecutive days with accelerating speed (J). To analyze locomotion open field test was performed showing total distance traveled and speed during a 5 minute period (K, L). To test anxiety time in the center of the open field (M) and time spend in the open arms of the elevated plus maze (N) was analyzed. Denotation of significance levels: * = p<0.05, ** = p<0.001 and *** = p<0.0001.