Rapamycin reduces proinflammatory cytokine production induced by HRV infection.

<p>BEAS-2B cells were infected with RV-16 at 1×10⁷ TCID₅₀/ml for 1 h (following which supernatants were replaced with media) (A, B), or stimulated with poly(I:C) at 10 µg/ml (C, D). Cells were then immediately treated with rapamycin at the indicated concentrations and cultured for 24 h. Cell-free supernatants were collected, and release of CXCL8 (A, C) and CCL5 (B, D) were measured by ELISA. Data shown are mean ± SEM of <i>n</i> = 3, where each replicate was performed on a separate passage of cells. Significant differences are indicated by *, <i>p</i><0.05 and **, <i>p</i><0.01, analysed by two-way ANOVA with Bonferroni's post-test.