Dual knockdown of p85α and p85β modestly effects HRV-induced CXCL8 release.

<p>BEAS-2B cells were either left untransfected (Utx), transfected with a non-targeting scrambled control siRNA (Scr) or siRNA targeting p85α and p85β at 100 nM. After 48 h transfection, cells were infected with RV-1B at the indicated TCID₅₀/ml for 1 h (following which supernatants were replaced with media) (B, C), or stimulated with poly(I:C) at the indicated concentrations (D, E). Cells were then cultured for 24 h. (A) Whole-cell lysates were analysed by western blot using Abs specific to p85α, p85β or actin. Data are representative of 3 (for HRV) and 2 (Poly(I:C)) independent experiments, each replicate was performed on a separate passage of cells. CXCL8 (B, D) and CCL5 (C, E) release was measured by ELISA. Significant differences (for HRV) are indicated by *, <i>p</i><0.05 analysed by two-way ANOVA with Bonferroni's post-test. LD = limit of detection.