PI3K inhibitors LY294002 and PI-103 reduce viral replication.

<p>BEAS-2B cells were infected with RV-1B (A) or RV-16 (B) at 1×10⁷ TCID₅₀/ml for 1 h (following which supernatants were replaced with media). Cells were then immediately treated with DMSO control, or with LY294002 (10 µM), PI-103 (0.5 µM) or a combination of four isoform-selective class I (C1) PI3K inhibitors [PIK-75 (α) (0.1 µM), TGX-221 (β) (0.1 µM), IC87114 (δ) (5 µM) and AS605240 (γ) (0.1 µM)]; and cultured for 24 h. RNA was extracted and reverse transcribed for qPCR analysis of HRV RNA expression, with data presented as the percentage of viral replication (normalised to DMSO control). Data shown are mean ± SEM of <i>n</i> = 4, where each replicate was performed on a separate passage of cells. Significant differences are indicated by *, <i>p</i><0.05, analysed by one-way ANOVA with Tukey's post-test.