<i>CS</i> silencing increases drug sensitivity in ovarian cancer cells.

<p>(<b>A</b>) Protein level of CS was examined in <i>CS</i>-silenced SKOV3 and A2780 cells. (<b>B</b>) SKOV3 and A2780 cells were treated with <i>CS</i> siRNA or negative siRNA for 48 h, then cells were treated with different concentrations of DDP for another 24 h. Cell viability was measured after incubation with CCK8 for 1.5 h. (<b>C</b>) 48 h after <i>CS</i> siRNA transfection, SKOV3 and A2780 cells were treated with 1 µg/ml DDP for 12 h and 1 h, respectively. Then cells were plated in 6-well plates, colonies were stained and counted after incubation for 8 days. Results shown were representative of three independent experiments. (<b>D</b>) SKOV3 and A2780 cells were treated with <i>CS</i> siRNA for 48 h, ERCC1 and γ-H2AX protein levels were compared between NC and siCS1078 group with β-tubulin used as a loading control. Mean ± SEM. *<i>P</i><0.05, **<i>P</i><0.01 and ***<i>P</i><0.001.