EZH2/ΙΤGα2-regulated cofilin activity controls cell morphology and migration.

<p>A. F-actin was stained with phalloin conjugated to a fluorophore (red) in HCT116 and HCTshEZ-2 cells cultured in matrigel. Representative phase contrast microscopy pictures of the same cultures are also presented for each cell line. Arrows indicate long F-actin molecules. Scale bar 20 µm. B. Analysis of relative amount of F- and G-actin in CRC cells with high (HCT116 and HCT-shpSUP) and silenced (HCTsh-EZ-2a/b) EZH2 protein levels. Intensity of WB bands has been quantified and the ratio is calculated in arbitrary units. Representative blot of F- and G-actin is also shown for each cell line. Student T-test was used to evaluate the statistic significance (*** = p-value<0,01) between HCTsh-EZ-2a/b cells and HCT116 cells. C. Migration ability of CRC cell lines transfected with siITGα2. D. WB analysis of p-cofilin levels in CRC cells with high (HCT116 and HCT-shpSUP) and silenced (HCTsh-EZ-2) EZH2 protein treated with ROCK1 inhibitor (Y27632) compared to the untreated cells. E. Migration ability of CRC cell lines treated with ROCK1 inhibitor (Y27632) compared to the untreated cells.