Amino acid properties under positive (red) and negative (green) selection in vertebrate <i>Hh</i> coding sequences.

<p>Two different signifcance levels are shown: (Z-score > |1.64|) to detect significant selective signatures and (Z-score > |3.09|) to detect strong selective signatures. Amino acid properties are classified as chemical (C), structural (S) and other (O), according to da Fonseca <i>et al.</i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0074132#pone.0074132-daFonseca1" target="_blank">[54]</a>.