<p>(A) PCR bands of miR-150 and miR-155 were detected by both TSLP and USLP methods. The PCR products were run on 3% agarose gel in 1X TBE stained with ethidium bromide. (B) Melting curve of the TSLP and USLP methods for detecting synthetic miR-155.</p
<p>(A) Schematic drawing of two-color detection experiments. Each D-probe can hybridize and be ligat...
There are relatively few protocols described for the in situ detection of microRNA (miRNA) and they ...
<p>Cross reaction (%) of each let-7 miRNAs by specific pincer probe real-time PCR assays.</p
<p>Curve of the new method assay was a straight line (R<sup>2</sup> = 0.993) with a slope of 3.63 (P...
<p>Synthetic miR-155 was diluted by over four orders of magnitude and qRT-PCR using the TSLP and USL...
<p>Light blue lines indicate total RNA. Arrowheads indicate directions of polymerization. 8 random s...
RT-qPCR is the accepted technique for the quantification of microRNA (miR) expression: however, stem...
<p>Total RNAs were reverse transcribed into cDNA using S-Poly(T) RT primer, oligo(dT) RT primer and ...
<p>Amplification plot of mmu-mir-1 in range from 10 ng to 10<sup>–</sup>3 ng input mouse heart total...
<p>The comparative ΔΔCT method was used for the qRT-PCR experiments and miR159 was selected as the r...
<p>Expression of miR-150 was decreased nearly 10-fold with prolonged incubation time (incubated at 0...
<p>Comparison of TSP endpoint detection by analysis of PCR product sizes on ethidium bromide-stained...
The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the ...
<p>A) Melting curve of the 11 reference genes showing a single pick (each including three technical ...
<p>Representative agarose gels showing PCR-RFLP analysis of different single nucleotide polymorphism...
<p>(A) Schematic drawing of two-color detection experiments. Each D-probe can hybridize and be ligat...
There are relatively few protocols described for the in situ detection of microRNA (miRNA) and they ...
<p>Cross reaction (%) of each let-7 miRNAs by specific pincer probe real-time PCR assays.</p
<p>Curve of the new method assay was a straight line (R<sup>2</sup> = 0.993) with a slope of 3.63 (P...
<p>Synthetic miR-155 was diluted by over four orders of magnitude and qRT-PCR using the TSLP and USL...
<p>Light blue lines indicate total RNA. Arrowheads indicate directions of polymerization. 8 random s...
RT-qPCR is the accepted technique for the quantification of microRNA (miR) expression: however, stem...
<p>Total RNAs were reverse transcribed into cDNA using S-Poly(T) RT primer, oligo(dT) RT primer and ...
<p>Amplification plot of mmu-mir-1 in range from 10 ng to 10<sup>–</sup>3 ng input mouse heart total...
<p>The comparative ΔΔCT method was used for the qRT-PCR experiments and miR159 was selected as the r...
<p>Expression of miR-150 was decreased nearly 10-fold with prolonged incubation time (incubated at 0...
<p>Comparison of TSP endpoint detection by analysis of PCR product sizes on ethidium bromide-stained...
The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the ...
<p>A) Melting curve of the 11 reference genes showing a single pick (each including three technical ...
<p>Representative agarose gels showing PCR-RFLP analysis of different single nucleotide polymorphism...
<p>(A) Schematic drawing of two-color detection experiments. Each D-probe can hybridize and be ligat...
There are relatively few protocols described for the in situ detection of microRNA (miRNA) and they ...
<p>Cross reaction (%) of each let-7 miRNAs by specific pincer probe real-time PCR assays.</p