Wildtype mice have no callosal astrocytosis or microgliosis 12 months after 2-hit frontal injury.

<p>Wildtype mice (n = 8–10 per group) received a 2-hit frontal impact injury or sham treatment, underwent behavioral testing 2–6 weeks later, and were analyzed histologically 12 months after the initial injury. Coronal brain sections were immunostained for GFAP or Iba-1(A, B) Representative images (A) and quantitation (B) of GFAP immunoreactivity in the corpus callosum. (C, D) Representative images (C) and quantitation (D) of Iba-1 immunoreactivity in the corpus callosum. Sh, Sham, H, Hit. Scale bar: 80 µm. Data are means ± SEM.