Induction of A20 upon IL-1β or TNFα treatment.

<p>(A) mRNA of primary hepatocytes treated with IL-1β (20 ng/ml), TNFα (25 ng/ml), FasL (50 ng/ml) or the combination was isolated after the indicated time. The gene expression of A20 was analysed by qRT-PCR. Four independent experiments ± s.d. are displayed. (***p<0.001, IL-1β versus TNFα treated cells at the corresponding time point). (B/C) A20 protein levels in wt hepatocytes treated with 20 ng/ml IL-1β, 25 ng/ml TNFα, 50 ng/ml FasL or the combination is shown for the indicated times. α-tubulin was used as a loading control. A representative immunoblot is shown (n = 3).