Nur1-Clb2 delays Cdc14 release.

<p><b>A</b>. Quantification of Cdc14 release, in experiments performed as in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004907#pgen-1004907-g003" target="_blank">Fig. 3</a>, relative to spindle length. At least 15, but typically more, cells were counted for each spindle length category. The mean and standard deviation from 3 independent experiments is shown. A logistic regression analysis showed that Cdc14 release timing in the Nur1-Clb2 strain was significantly delayed. <b>B</b>. Serial dilution assay demonstrating rescue of cell survival of Nur1-Clb2 cells by dominant active Cdc14^TAB6-1. <b>C</b>. Western blot samples taken of the cells in <b>B</b>, before they were plated, shows that Cdc14^TAB6-1 did not alter the Nur1-Clb2 phosphorylation status. <b>D.-F.</b> Active Cdc14 rescues mitotic progression of Nur1-Clb2 cells. As <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004907#pgen-1004907-g003" target="_blank">Fig. 3A-C</a>, but strains carried the <i>CDC14^TAB6-1</i> allele.