Effectiveness of intranasal (IN) and intramuscular (IM) <i>L. amazonensis</i> antigens (LaAg) vaccination against subsequent infection with <i>L. braziliensis.</i>

<p>Golden hamsters (6–8 per group) were vaccinated with either 10 µg of LaAg intranasally (•) or 20 µg of LaAg intramuscularly (▴) followed by a booster vaccination 14 days later. Controls received PBS (□). Two weeks after the booster vaccination, animals were infected in the paw with 1×10⁵<i>L. braziliensis</i>. (A) Lesion thickness was scored on the indicated days. Means ± standard errors of the means (SEM), * p≤0.05, ** p≤0.01. (B) On day 114 post-infection, the lesion thickness of individual feet was shown as individual values, with the horizontal bars representing the median values. Data are from four independent experiments.