Neutralization profiles of proviral variant strains with serum from animals experimentally infected with EV0, EV6, EV13, or EVCon.

<p>Variant proviral strains were tested for <i>in vivo</i> pathogenecity through experimental infections of ponies. Serum from the infections was utilized here to test for immunogenicity and characterize the variant Envs. At six-months post-infection serum from two representative animals (labeled A,B) was tested in our standard neutralization assay. The mean reciprocal dilutions of serum which neutralized 50% of input EV0, EV6, EV13, or EVCon as measured in an infectious center assay are presented. The dashed line denotes the cut off (≥25) value for valid 50% neutralization titers.