Peptide binding affinities for human MHC II proteins.

<p>IC₅₀ values are plotted as cognate wild type and variant pairs, where lower IC₅₀ values correspond to higher affinity binding with human MHC II. The slope of the connecting lines are a relative measure of deimmunizing efficacy, where larger positive slopes indicate a greater fold decrease in affinity relative to wild type. Lines with negative slopes indicate a mutation that enhanced MHC II binding. Shading indicates binding strength by category: strong (IC₅₀<1 µM, dark grey), moderate (1 µM≤IC₅₀<10 µM, medium grey), weak (10 µM≤IC₅₀<100 µM, light grey), or non-binding (IC₅₀ ≥100 µM, white).