Effect of AAL and AAS treatment on nitrite production in infected BALB/c mice.

<p>Macrophages isolated from peritoneal cavity of differently treated, infected and naïve mice and plated (1× 10⁶ cells/well), stimulated with SLA (A) at 10µg ml⁻¹, unstimulated (B) and re-stimulated (C) with AAL and AAS for 48 h. Nitrite levels in macrophage culture supernatant of indicated treatment groups were determined by the Griess assay. Data represent the mean ± SE for five animals per group. Data were tested by ANOVA. Differences between means were assessed for statistical significance by Tukey's test (***, <i>P</i> ≤ 0.001).