Effect of FA on cell motility and biofilm formation by <i>P. protegens</i> Pf-5.

<p><b>A</b>. Cultures of <i>P. protegens</i> Pf-5 (wild type) were grown aerobically overnight to OD = 3 in KMB medium. An aliquot was spotted onto the centre of plates containing 20 fold diluted KMB medium supplemented or not with FA and solidified with various concentrations of Bacto-Agar, depending on which motility was to be evaluated. After 24 h of incubation at room temperature, the distance of the migration front from the point of inoculation was measured. <b>B</b>. Cultures of <i>P. protegens</i> Pf-5 were grown in 96-well polystyrene plates containing E₂ glucose minimal medium supplemented or not with FA. After 24 h of incubation, biofilm formation was assessed by the determining the proportion of attached and non-attached bacteria (A₅₉₅/OD₆₀₀). In all cases, error bars indicate the standard deviation of the mean. The asterisk (*) denotes significant differences (P<0.05) using ANOVA.